ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (2): 332-338.doi: 10.11843/j.issn.0366-6964.2015.02.022

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The Protection of Vitamin E on Quality and Integrity of Mice Semen in vivo

ZHAO Xian-lin1,CAO Shao-jie2,ZHAO Chun-ping1,HAO Rui-jie1,GUI Lin-sheng1,ZAN Lin-sen1*   

  1. (1.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,China;2.Weinan Vocational & Technical College,Weinan Testing & Inspection and Research Center of Agricultural Products and Food,Weinan 714000,China)
  • Received:2014-11-02 Online:2015-02-23 Published:2015-02-23

Abstract:

This study aimed to investigate the effects of vitamin E added in diet on sperm DNA integrity and antioxidant activities of semen in mice.50 mice divided into 5 groups were fed in the experiment,in which vitamin E was added in diet with the different content of 0(Control),4,8,12 and 16 g•kg-1,respectively.The mice were killed by cervical vertebra dislocation and dissected after 30 days when semen was obtained from epididymis and seminiferous tubules.The sperm motility,density,deformity rate and DNA integrity were detected by single cell gel electrophoresis(SCGE) assay and test kits,as well as the antioxidant activities including superoxide dismutase (SOD),glutathione peroxidase (GSH),glutathione reductase (GR) and catalase (CAT).The percentage of “grade 0” sperm in diet supplemented with 12 g•kg-1 vitamin E was significantly higher than that of other treatment groups (P<0.05).The sperm motility was significantly improved (P<0.05) and the levels of GR and SOD in seminal plasma were significantly higher (P<0.05) by the supplementing with 8-16 g•kg-1 vitamin E in diet than the control,respectively.The CAT activity in seminal plasma reached the highest level with supplementation of 12-16 g•kg-1 vitamin E in diet,when compared to the others (P<0.05).In conclusion,we could infer that 12 g•kg-1 vitamin E added could obviously improve the sperm DNA integrity and the antioxidant activities of CAT,GR,GSH and SOD in seminal plasma.

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